endothelial cell culture medium ecm  (ScienCell)

Journal: Biomedicines

Article Title: Evaluation of Platelet Lysate-Based Medium and Protein Substrate for HUVEC Culture and Expansion

doi: 10.3390/biomedicines13051187

Figure Lengend Snippet: HUVEC phenotype with mixed medium and ECGM- Promocell ® with and without PL protein substrate. Data are represented in the graph as the expression level of surface markers CD31, CD144, CD146, CD34, and HLA-DR. Evaluation of the HUVEC phenotype was carried out in cultures with different media modifications (mixed medium and ECGM- Promocell ® with and without PL protein substrate). It was observed that CD31, CD144, and CD146 markers are expressed in more than 90%, while CD34 and HLA-DR markers are expressed in less than 10%. These results confirm that the profile of endothelial cells remains unaffected by any medium modification used.

Article Snippet: Commonly used commercial culture media include endothelial cell growth basal medium (EBM, Lonza ® ; Basel, Switzerland) [ , ], complete endothelial cell culture medium (ECM, Endothelial Cell Medium; ScienCell; Carisbad, CA, USA) [ , ], vascular cell basal medium (ATTC ® ; Manassas, VA, USA) [ ], and endothelial cell growth medium for large, medium, and microvascular tissue vessels (ECGM, PromoCell ® ; Heidelberg, Germany) [ , , ].

Techniques: Expressing, Modification